The Movie House at Dublin Road in Belfast is to close in May after the site was bought by software company Kainos.
It’s hoped the 33 staff members will be redeployed to the other cinemas.
Kainos has bought the site which has planning permission for an office building.
Michael McAdam, Movie House Cinemas, managing director, said the cinema has been based at Dublin Road for 12 years, but added that he has seen a big shift in cinema attendance out of Belfast city centre to suburban locations, which offer free parking.
“It was a very difficult decision whether to sell or reinvest in the site. It is with a heavy heart that we decided on the latter and we wish Kainos every success with the redevelopment of the site for their new headquarters.
“Dublin Road Movie House is due to close at the end of May and we hope to be in a position to offer jobs to the 33 full and part time staff members at our other cinemas at that time,” he added.